Protein Purification Kits, columns, gels, reagents, enzymes, inhibitors, and other assorted products for use with protein purification procedures. Spin columns are available in 10mL, 5mL, 3mL, 1mL, and 100L volumes. PureCube 1-step batch spin columns are available in two sizes: Mini for expression trials, small-scale screening, and other small-volume purification needs; or Midi Plus for volumes of up to 20 mL. Looking for spin columns? These spin columns are constructed of polypropylene with a polyethylene FRIT. MONOSPIN TRYPSIN HP 30/PK KEEP COOL. 2 x 10 kDa MWCO Ultrafiltration Spinners. These spin columns for DNA purification can be used both as a prefiltration step, to lower particulates, and as a support filter in combination with the other filters. The columns fit into most microcentrifuges. Add to cart. # 786-798 is a spin format that is ideal for the rapid, small scale purification of IgG. Nickel-NTA Agarose Resin, 1ml Spin Column quantity. This kit provides a column to deprotenise small amount of protein . Elution conditions formatted for both electrophoresis and LC-MS analysis workflows. Here, we show that silica paper spin columns and borosilicate multi-well . If larger resin volume is required, then spin duration may increase. Ion Exchange. Proteus purification kits use a unique technology that combines protein separation by gravity flow with the speed and ease-of-use of spin columns. NOTE: Ensure centrifuge is properly balanced if only one spin column is used. Collect fractions. 8 x 50 ml Centrifuge Tubes. Biocomma is ISO9001 . Protein Purification Columns. Thiomersal. Like all Ni-NTA matrices, Ni-NTA spin columns can be used for one-step protein purification under native or denaturing conditions. The Ni-NTA Spin Kit is a complete kit for spin purification of His-tagged proteins. Plug Insertion Tool. 1 x 250 ml of 1 x Buffer G. 1 x 125 ml of 1 x Buffer B2. Products. View . You can purify and extract DNAs and RNAs that includes Genomic DNA, Plasmid DNA, Viral DNA/RNA, DNA fragments with the correct filter. Protein Extraction and Purification. 50 x 0.5 mL Columns. Prepacked Chromatography Columns by Resin Type. Spin Columns and Gravity-flow Columns. 12 x Spin Columns. This technology allows one-step purification of almost any His-tagged protein from any expression system under native . The QIA express Ni-NTA Protein Purification System, including the Ni-NTA Superflow Columns, is based on the remarkable selectivity of patented Ni-NTA (nickel-nitrilotriacetic acid) resin for proteins containing an affinity tag of six or more histidine residues the His tag. Protein Purification Spin Columns and Gravity-flow Columns PI69725 Print Thermo Scientific Pierce Spin Columns - Snap Cap Perform small-scale affinity methods, such as immunoprecipitation assays with agarose beads using these empty spin columns and cups (5 to 500L). Suitable for purifying. Reagents in the Kit. The spin columns contain a unique immobilized metal affinity chromatography resin that binds phosphopeptides. Biocomma Limited, founded in 2006, is a leading manufacturer of filtration, sample preparation, oligo synthesis and nucleic acid purification products, based on its two technology platforms of porous plastic filters and separation materials. 0.01%. Cart. Compare this item. The 2 ml spin columns are recommended for low-speed centrifuges with swinging buckets. These spin columns are uniquely designed, with a snap off end converting to a closure for the Regulatory. SPECIFICATIONS Sinter Type: Ultra High Density Polyethylene Construction: Polypropylene Pore Size: 0.1-0.2 m low protein binding PVDF Maximum Vol: Mini: 600 l Midi: 20 ml Maximum g force: Spin columns each containing 0.5 ml of TALON-NX, an immobilized metal affinity chromatography resin for the purification of recombinant his-tagged proteins under native or denaturing conditions. Spin columns enhance the process of nucleic acid purification making it a lot faster. Compare Product No. Concentrate the protein with VIVASPIN 15R Ultrafiltration spin column to reduce the volume to ~1 ml . By using recharged or homemade filter paper-based spin column along with leftover of commercial kit or homemade buffer solutions, the total expense for purification can be as low as $0.7 per DNA sample (with RNase as the major contributor to the final cost), and less than $0.5 per RNA sample. 635603. Protein A HP SpinTrap contains Protein A Sepharose High Performance, which has high protein binding capacity, and is compatible with all buffers commonly used in antibody purication. The columns are designed to fit microcentrifuge tubes, 15mL conical tubes and 50mL conical tubes, respectively. The spin columns conveniently fit in to common centrifuge tubes for easy washing and collection (Mini: 2 ml centrifuge tubes, Midi: 50 ml centrifuge tubes). It can be automated on the QIAcube Connect. These columns have the capacity to bind up to 250 g of phosphopeptide and can accommodate up to an 850 l sample volume. Details. 2 x Protein A Spin Column Plugs. Protein Purification. So can I use same buffer by adding imidazole for chromatographic purification of protein on Ni column? Thermo Scientific NAb Spin Columns are available in three sizes. Images - 10kD Spin Column (ab93349) Representative 10kD Spin Column (ab93349). in multiple formats, including conventional gravity-flow chromatography, spin column, and FPLC. Ease-of-use: One-step sample concentration in a single tube, for minimal sample handling and reduced sample loss, as well as pH compatibility over a broad range - pH 1 to 9. Protein Biology. By condensing the protein preparation workflow, the Amicon Pro system eliminates the need for multiple sample transfers, thereby minimizing protein loss. The spin columns are made to hold different volumes in order to accommodate a wide variety of sample sizes. 2 x Spin Columns. 1 - 30 of 129 results. proteomics protein-purification: 10595: Nickel Ni-NTA Agarose Resin, 3ml Spin Column $ 220.00 They allow rapid purification of proteins from crude cell lysates under either native or denaturing conditions. Fast binding kinetics and high capacity provide high yield. They are provided with inserted frits (mesh size 10 m), screw caps, and snap-off plugs . AHN myTube SC spin columns for DNA purification with polyethylene filters are supplied with 20-60 m pores and are suitable for coarse filtration with the aim of particle removal. Manufacturer: Thermo Scientific 69725 Catalog No. Abstract. The G-Trap FliQ FPLC columns are housed in medical grade polypropylene and are resistant to commonly used column chromatography reagents. These spin columns are available in the following sizes: <0.1, 1, 2, 3, 5 and 10 ml. 4 x 2.2 ml Centrifuge Tubes. Protease Inhibitors and Phosphatase Inhibitors (2,917) Protein Purification Reagents and Kits (1,209) Spin Columns and Gravity-flow Columns (154) Gel Filtration Media and Columns (108) The Car9 affinity tag is a dodecameric silica-binding peptide that can be fused to the N- and C-termini of proteins of interest to enable their rapid and inexpensive purification on underivatized silica in a process that typically relies on l-lysine as an eluent. About Biocomma. The flow regulator substantially increases the yield and purity of the purified target protein or antibody. Add to cart. MINI Kits for purification of up to 1 mg of protein in 15 minutes Protein A columns are available for use in the laboratory to aid in any research and development that requires purification of IgG from complex media. Whatever your protein purification needs, we have a comprehensive range of small- and large-scale protein isolation kits for purification of His- or Strep -tagged recombinant proteins under native or denaturing conditions. Reagents in the Kit. The Proteus purification spin column kits for affinity purification of His-tagged proteins (Ni-IMAC) and antibodies (Protein A and G). Purification columns and filter spin columns that are prepacked or designed to be packed with desalting resins, agarose beads, and other affinity supports for use in centrifuge/microcentrifuge or gravity-flow chromatography procedures. Hide. 11274015001: Purification Use at an assay dependent concentration. With protein purification columns, protein purification chromatography and magnetic agarose beads, you are sure to find an . Includes ready-to-use pre-packed Ni spin columns and all required buffers for purification Purification of 1 mg His-tagged protein per column in as little as 15 minutes High specific binding of His-tagged proteins yielding purities of >95% Strong nickel ion binding provides excellent resistance to EDTA and reducing agents. The 0.8mL, 2mL and 10mL centrifuge columns are filled respectively with 0.2mL, 1mL and 5mL of Protein A, G, A/G or L beaded agarose resin. Each column containing 1 mL of the immobilized protein resin enables quick purification of 34 mg of human IgG from 0.5-2 mL of serum or other sample. All steps in the purification procedure are fully automated up to . The kit is supplied with 3ml Pearl IgG Purification Resin, IgG Isolation Buffer and 20 spin columns. Spin columns can contain a wide variety of filters and these filters come with distinctive bore sizes like 2/3/4/6/8 layers. Thiomersal. Ni-NTA resins are commonly chosen for His-tagged-protein purification because of the four metal-binding sites on the chelate, which allow for high-binding capacity and low-metal ion leaching. All Photos (1) Quick Spin Columns for radiolabeled RNA purification. 4 x 10 kDa MWCO Ultrafiltration Spinners. Protocols. Features include: For simple, small-scale antibody purification or enrichment of specific proteins in prepacked, single-use spin columns that are ready to use. Empty Spin Columns are ideal for rapid protein purification from small sample volumes. A spin column doesn't dilute the sample, but is not as efficient at salt removal. Features: Proteus Protein A & G Spin Column Kits A unique aspect of Pro-Chem's invention involves a proprietary flow regulator which is designed to control the flow rate of the sample through the agarose column. Up to 1 mg of His-tagged protein can be purified in 5 minutes and eluted in as little as 100 l of His-Elution Buffer. Features: Tubing material: polypropylene Frit material: ultrahigh molecular weight polyethylene (UHMW-PE) Column volues: 2 mL, 50 mL Order information: SpinTrap columns are prepacked, single-use spin columns for simple, small-scale purification of antibodies or enrichment of specific proteins. This makes them ideal for spin column protein purification, including applications requiring maximum protein and enzyme yields such as tissue culture media filtration, cold sterilisation and biological fluid filtration amongst others. The columns fit all standard microfuges and allow you to process multiple samples in parallel. Description SDS Pricing; 11273990001: Sephadex G-25, fine: Expand. biocomma empty spin protein purification columns, work well with centrifuge rotors, is a very economical and convenient choice to process a large number of samples in a very short time. Gravity & Spin. Each pre-filled microcentrifuge spin column of the immobilized protein resin enables quick purification of 100 to 1000 g of IgG from 25 to 500 L of serum or other sample. Sodium Azide (NaN 3) Resin contains 0.01%. Filters per page . Protein Purification Columns. . Then I pre-equilibrated the column using sodium acetate pH 4.5 (3 washes). The 3, 5 and 10 ml spin columns can also be used for gravity flow purifications. The one-step procedure allows purification of up to 300 g 6xHis-tagged protein per column in as little as 15 minutes. Pre-Equilibration 1. 4 x Protein G Spin Column Plugs. After being released by proteolytic cleavage, Mgm101 is separated from MBP by cationic exchange chromatography. The Thermo Scientific Protein A Plus Agarose NAb Spin Columns are convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. See more specifications for empty spin protein purification columns. Protein Ark's Proteus mini purification spin columns are designed for small scale protein purifications such as those required for expression trials, solubility determination tests, screening, tittering and scouting studies.
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